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. Author manuscript; available in PMC: 2020 May 4.
Published in final edited form as: Methods Enzymol. 2012;507:199–228. doi: 10.1016/B978-0-12-386509-0.00011-9

Table 1.

Preparation of reagents for qPCR analysis

Component Volume per 30-μl reaction Final concentration
2X qPCR master mix 15 μl 1X
Forward primer 0.15 μl 500 nM
Reverse primer 0.15 μl 500 nM
Probe 0.06 μl 200 nM
Water to 25 μl (to 20 μl for std.
curve)		 —