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. Author manuscript; available in PMC: 2020 May 4.
Published in final edited form as: Methods Enzymol. 2012;507:199–228. doi: 10.1016/B978-0-12-386509-0.00011-9

Table 2.

Time and temperature parameters for qPCR amplification

Initial denaturation and enzyme activation 1 cycle 95°C x 10 min
Amplification 40 cycles 95°C x 15 sec
60°C x 1 min