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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Gastroenterology. 2019 Dec 14;158(5):1417–1432.e11. doi: 10.1053/j.gastro.2019.12.010

Figure 4.

Figure 4.

IL22 enhances the expression levels of EMT-related transcription factors. (A) Treatment of PDAC cells with high-dose IL22 results in increased ZEB1, vimentin, and CDH2 and decreased CDH1. (B) After 24-hour incubation in IL22, mRNAof EMT-related transcription factors was detected by qRT-PCR (P < .5 relative to untreated for all genes). (C) Chromatin immunoprecipitation assay identified the association of Stat3 with the EMT transcription factor TWIST1 upon IL22 activation (n = 3; *P < .05). (D) Treatment with a JAK/STAT inhibitor abrogated IL22-mediated Stat3 phosphorylation by immunoblot. (E) Immunofluorescence showed increased protein expression of vimentin (red) and decreased CDH1 (green) after 48 hours of culture with IL22.This was blocked by inhibition of JAK/STAT (n = 5; scale bars, 20 μm). DAPI, 4′,6-diamidino-2-phenylindole.