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. 2020 May 1;34(9-10):637–649. doi: 10.1101/gad.333864.119

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© 2020 Yue et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 6. — Phendione-induced growth inhibition is ATM dependent. (A,B) Colony formation assay of A375 (A) and A375 BRAFi-resistant (B) cells treated with phendione or phendione in combination with ATMi (5 µM) for 3 h. Data are shown as mean ± SD of three independent experiments. (**) P < 0.01; (***) P < 0.001, by two-way ANOVA with Sidak's multiple comparisons test. (C,D) Synergy maps for A375 (C) and A375 BRAFi-resistant (D) cells showing the effect of combined treatment with phendione and ATMi at indicated concentrations for 96 h. The 2D synergy matrix was generated with SynergyFinder software using HAS model. (E) Immunoblots of A375 and A375 BRAFi-resistant cell lysates after treatment with phendione, pyrene (3 µM), or vehicle for 3 h in the presence or absence of ATM inhibitor (5 µM). (F) Immunoblots of A375 and A375 BRAFi-resistant cell lysates after 3 d of siRNA transfection as indicated. The cells were treated with vehicle or phendione for 3 h before collection.