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. 2020 May 1;34(9-10):701–714. doi: 10.1101/gad.335281.119

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© 2020 Beebe et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — dERR functions in the adult fat body to maintain lipid stores. (A,B) Whole-animal homogenates were prepared from controls (blue) or flies with adult-specific RNAi targeting dERR (red) or Pfk (green) in either the fat body (cg-GAL4, Tub-GAL80^ts) or intestine (Myo1a-GAL4, Tub-GAL80^ts). These samples were analyzed for glucose (A) and triglyceride (B) levels. Animals were maintained for the first 11 d of development at 18°C and subsequently transferred for the remainder of the experiment to 29°C. Data were normalized to the controls. Animals were fed a normal diet; n = 9–29 independent samples with five animals per sample. (***) P ≤ 0.001; (**) P ≤ 0.01; (ns) P > 0.05. (C,D) Bright-field images of adult fat bodies stained with Oil Red O to detect neutral lipids. Samples were collected from 6-d-old controls (C) or animals with adult-specific RNAi targeting dERR in the fat body (cg-GAL4, Tub-GAL80^ts) (D). Scale bar, 50 µm.