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. 2020 May 1;34(9-10):621–636. doi: 10.1101/gad.332577.119

Figure 5.

Figure 5.

Ptf1a enhancer mutants have increased sensitivity to itch inducing pruritogens. (A,B) Quantification of the number of scratch bouts in 30 min after injection of chloroquine (A) or histamine (B) at the neck reveals increased sensitivity to itch in the AR-DNT1 mutants relative to WT littermates. (C–J) Thermal sensitivity behavior tests including Hargreaves (C), hot plate (D), and cold sensitivity (E), von Frey testing for mechanical sensitivity threshold (F), acute chemical pain, Capsaisin induced (G), the rotor rod (I), and the balance beam (J) showed no significant difference in the AR-DNT1 mutants relative to WT. (H) AR-DNT1 mutants do show increased sensitivity to phase II nocifensive formalin-induced pain but not phase I acute pain. Each data point represents a biological replicate (N); error bars indicate SEM. Student's t-test (A–G) or two-way ANOVA (H–J) was used to determine significant differences relative to WT littermate controls, N-values and P-values are as indicated. See also Supplemental Fig. S5.