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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Res. 2019 May 2;79(11):2892–2908. doi: 10.1158/0008-5472.CAN-18-3026

Figure 2: ARF6-GTP upregulates PI3K expression and AKT activation.

Figure 2:

(a) RNA sequencing of mouse tumors reveals differential expression between melanomas from control (n=4) and Arf6Q67L mice (n=6). Left panel = heat map of relative expression shown, range of 2 to −2 (log2). Right panel = Upregulated gene biosets in ARF6Q67L tumors. * = gene sets that include Pik3r1. (b) ARF6Q67L induces 1.4503-fold increase in Pik3r1 expression in tumors. Unpaired t-test, two-tailed. (c) In mouse tumor cell lines derived from a distinct group of mice from those sequenced, ARF6Q67L increases both p85 and p110 PI3K protein levels. Ratio paired t-test, two-tailed. HA immunoblot detects ectopic HA-tagged ARF6Q67L. (d) Heatmap representing differences in signaling protein levels detected with reverse phase protein array. Columns represent individual mouse tumors. Unpaired t-test, two-tailed.