Table 21.

Reaction system to digest the PCR products

Component	For heavy chain (μL)	For kappa chain (μL)	For lambda chain (μL)
Overlapping product	50	50	50
10× Tango buffer	20	10	20
EcoRI	2	–	2
Xhoi	2	5	2
KpnI	–	5	–

ddH2O	26	30	26
Total	100	100	100