(A and B) Significant temporal order preplay during Pre-Run sleep (A) and replay during Post-Run sleep (B) of place cell sequences in Run for frames with at least 15 cells (template matching method). (Insets) Area under curve (AUC) (preplay, A, or replay, B, minus shuffle) for frames with 6 or more cells. Similar proportions and AUCs are obtained for frames with a minimum of 6–15 neurons (data not shown).
(C) Significance of preplay and replay and their similarity in strength are maintained across a wide range of within-frame number of participating place cells. p values reflect comparisons of preplay with its shuffle (blue), replay with its shuffle (green), and preplay with replay (red).
(D) Graphical representation of the lack of change in temporal order of neuronal firing from Pre- to Post-Run sleep frames with regard to Run.
For (A) and (B): ***p < 0.001.