Figure 5. Effect of combined treatment on MCF7 cell cycle.
MCF7 cells were seeded in 10-cm plates at a density of 2 × 106 cells per plate for 24 h. The cells were then treated with AEW541 (2 μM), or gemcitabine (5 nM), or both for 24, 48 and 72 h. At the end of the incubation period, cells were collected by trypsinization into their own medium to prevent loss of dead cells. Cells were fixed with 70% ice-cold ethanol, stained with propidium iodide and used for cell cycle and apoptosis analyses performed by FACS Calibur system. Graphs present the number of cells versus the amount of DNA, as indicated by the intensity of a fluorescence signal, upon each treatment.