Table 2.
Kinetic parameters of the spontaneous action potentials yielded under control conditions or in the presence of 300 nM or 3 μM nifedipine in WT and Cav1.3−/− cells
| Depol. rate (V/s) | Overshoot (mV) | HH Width (ms) | Height (mV) | Undershoot (mV) | Repol. rate (V/s) | Firing rate (Hz) | |
|---|---|---|---|---|---|---|---|
| WT (n = 6) | |||||||
| Control | 50.7 ± 10.9 | 31.0 ± 5.69 | 3.7 ± 0.7 | 81.4 ± 5.8 | −50.5 ± 2.9 | −29.1 ± 4.4 | 4.9 ± 0.9 |
| Nife 300 nM | 38.6 ± 11.2 | 31.0 ± 4.0 | 5.4 ± 1.2* | 72.9 ± 8.2 | −45.7 ± 3.8* | −18.9 ± 4.5* | 3.1 ± 0.8 |
| Nife 3 μM | 39.2 ± 13.2 | 29.4 ± 5.0 | 5.8 ± 1.2* | 73.2 ± 7.4 | −43.8 ± 3.7*** | −15.9 ± 3.9* | 1.6 ± 0.5** |
| Cav1.3−/− (n = 4) | |||||||
| Control | 46.6 ± 18.7 | 26.3 ± 7.9 | 3.3 ± 0.5 | 76.6 ± 10.5 | −50.4 ± 3.6 | −33.0 ± 5.5 | 4.5 ± 1.0 |
| Nife 300 nM | 40.3 ± 12.4 | 28.2 ± 6.6 | 3.8 ± 0.6* | 76.5 ± 9.0 | −48.3 ± 3.8*** | −26.5 ± 5.7# | 3.1 ± 0.8* |
| Nife 3 μM | 36.7 ± 8.2 | 29.0 ± 5.3 | 4.2 ± 0.5* | 75.2 ± 7.6 | −46.2 ± 3.9* | −20.6 ± 40.2*** | 1.2 ± 0.6* |
A spike was considered an action potential when the peak exceeded +10 mV. Data were obtained in two paired cultures of WT and Cav1.3−/− cells, using 1–2 mice of each strain. *p < 0.05; **p < 0.01; ***p < 0.005; # p < 0.001. No statistically significant differences were detected in the different parameters between WT and Cav1.3−/− cells.
Depol. rate: maximal depolarization rate; Overshoot: maximal potential; HH Width: half‐height width; Height: total amplitude; Undershoot: minimal potential; Repol. rate: maximal repolarisation rate; Firing rate: frequency of firing.