Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr;30(4):515–527. doi: 10.1101/gr.253211.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Casa et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 1. — Acute auxin-inducible degradation of STAG1 and STAG2. (A,B) Schematic of the CRISPR-mediated modification of STAG1 (A) and STAG2 (B) to add a mini auxin-inducible degron tag (mAID) and a mClover tag to the C termini of STAG1 and STAG2 in HCT116-CMV-OsTIR1 cells. (C,D) Addition of auxin leads to a degradation of STAG1-AID (C) and STAG2-AID (D) within 2 h without affecting the protein level of the respective other ortholog. (E,F) Visualization of the STAG1-AID (E) and STAG2-AID (F) degradation 12 h after auxin addition by immunostaining. (G,H) ChIP-qPCR with anti-EGFP antibodies (detects mClover) shows efficient depletion of STAG1-AID (G) and STAG2-AID (H) from cohesin sites in the H19/IGF2 locus.