Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr;30(4):515–527. doi: 10.1101/gr.253211.119

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Casa et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — Hi-C reveals different contributions of STAG1 and STAG2 to higher-order chromatin structure. (A,B) Differential maps of Chromosome 17 illustrating the chromatin contact changes after auxin-mediated degradation of STAG1 (A, STAG1-AID) or STAG2 (B, STAG2-AID). Examples of changes are encircled. Color key = Balanced (Knight-Ruiz) Hi-C contact frequencies. (C,D) Zoom-in for a region of Chromosome 13 into the contact maps for STAG1-AID (C) and STAG2-AID (D) cells. The maps for untreated cells (w/o auxin), treated cells (+auxin), and the differential interaction maps split in loss of interactions (blue) and gain of interactions (red) are shown. (E) Change of chromatin contact frequency relative to the separation of the contacting bins (25 kb) after STAG1 or STAG2 degradation for the examples of Chromosomes 6 and 17. (F) Aggregate peak analysis of STAG1-only peaks showing that a lot of the sites lose their respective contacts after STAG1 degradations while alternative contacts are formed. (G) Insulation plots showing the averaged Hi-C signals for all STAG1/STAG2 shared peaks as well as all STAG1-only and STAG2-only peaks in the untreated maps (w/o auxin). For the same peaks, the averaged differential Hi-C signals in the different maps are shown. The maps are presented with a bin size of 25 kb and 10 bins to the left and right of each binding site.