Characteristic Study ID	Measurement period		Study population	Intervention group	Control group	Overall effect	Comments
Bayne‐Smith 2004	Baseline		442 girls	160	154	‐	A Reflotron reflectance photometer measured total serum cholesterol.
	End of intervention			151	150	NS
Bush 1989	Baseline		431 boys and girls	180	175	P < 0.01	Nonfasting blood samples were taken using venipuncture Treatment effect favors intervention group but not statistically significant
	During intervention (year 1)			187	189	P < 0.02
	During intervention (year 2)			189	189	P = 0.418
	During intervention (year 3)			171	167	P = 0.432
	End of intervention (year 4)			177	180	P = 0.348
Luepker 1996	Baseline		4019 boys and girls	4.39	4.41	P = 0.64	Nonfasting blood samples were obtained via venipuncture and analyzed for total cholesterol.
	End of intervention (year 3)			4.15	4.14	P = 0.21
Martinez 2008	Boys	Baseline	1044 boys and girls	169	171	P = 0.48	12‐hour‐fasting blood samples were obtained under standardized conditions from the cubital vein in Vacutainer serum‐gel‐separator tubes. Samples were processed within 60 min of drawing
		End of intervention (9 months)		163	165	P = 0.54 1 (95% CI ‐2 to 4)
	Girls	Baseline		169	169	NS
		End of intervention (9 months)		165	163	P = 0.78 1 (95% CI ‐3 to 4)
Reed 2008	Baseline		514 boys and girls	4.3	4.5	NS	Intravenous samples were taken from the antecubital vein between 8:00 AM and 9:30 AM, after an overnight fast. A 10 mL sample was taken by a nurse and stored on ice in a serum separator tube. Blood was separated within 30 min and then stored at ‐80 °C
	End of intervention			4.1	4.3	NS
Simon 2004	Baseline		954 adolescent males and females	158	153	‐	Subjects are asked to fast for a minimum of 10 h before the blood sampling, which was performed in the morning, before the medical examination, with minimal venous stasis. All samples were processed within 3 h of sampling and the serum and plasma samples were aliquoted for immediate analysis or long‐term storage at ‐80 °C until assay
	End of intervention (year 4)			168	160	P = 0.15 3 (95% CI ‐1 to 6)
Walter 1988	Westchester	Baseline	3388 boys and girls	169	166	‐	Nonfasting blood samples were taken to measure total cholesterol
		End of intervention		167	166	P < 0.05
	Bronx	Baseline		174	170	‐
		End of intervention		171	168	NS
Walther 2009	Baseline		182 boys and girls	4.2	4.26	NS	Peripheral blood samples were obtained before the specific tests were performed in all pupils. Technicians were blinded for group assignments
	End of intervention (year 1)			4.19	4.12	P = 0.370 0.11(‐0.13 to 0.35)
Wang 2008	Baseline		316 boys and girls	166	161	NS	The biological measurements were made in a mobile laboratory that was brought to the school sites. Non‐fasting total cholesterol were measured with a Cholestech LDX system
	End of intervention			172	169	P = 0.94 0 (95% CI ‐6 to 6)
Young 2006	Baseline		210 adolescent girls	155	161	‐	‐
	End of intervention (1 year)			155	163	P = 0.43
CI: confidence interval; NA: not acknowledged; NS: not significant; mL: millilitres.