Table 4.
Overview of method validation parameters
| Imprecision (RSD), % |
||||||||
|---|---|---|---|---|---|---|---|---|
| Accuracy, % | intraday | interday | Mean REC, % (RSD, %) | Mean EE, % (RSD, %) | Mean ME, % (RSD, %) | Mean IS-nME, % (RSD, %) | ||
| Remdesivir | High QC level | 104 | 2 | 6 | 67 (6) | 66 (7) | 2 (2) | −10 (1) |
| Medium QC level | 100 | 1 | 6 | 67 (8) | 67 (11) | −1 (3) | −6.9 (3) | |
| Low QC level | 87 | 5 | 6 | 78 (4) | 67 (9) | 16 (7) | 3 (7) | |
| LLOQ | 118 | 10 | 12 | |||||
| Mean (RSD) | 102 | 4.5 | 7.5 | 71 (6) | 67 (9) | 6 (4) | −5 (4) | |
| GS-441524 | High QC level | 96 | 2 | 3 | 104 (6) | 99 (5) | 5 (4) | 1 (4) |
| Medium QC level | 102 | 6 | 4 | 99 (5) | 105 (17) | −3 (21) | −9.2 (10) | |
| Low QC level | 92 | 9 | 11 | 104 (10) | 112 (9) | −7 (10) | −10 (10) | |
| LLOQ | 81 | 9 | 14 | |||||
| Mean (RSD) | 93 | 6 | 8 | 102 (7) | 105 (10) | −2 (12) | −6 (8) | |
RSD, relative standard deviation; REC, recovery; EE, extraction efficiency; ME, matrix effect; IS-nME, internal standard-normalized matrix effect.