Fig. 5.

Efficacy of defatting cocktails in human steatotic PCLS. (A-J) PCLS were prepared from steatotic human liver samples, and after 1 h in primary culture they were treated with D-FAT, D-FAT with NSA, or vehicle for 24 h, and examined for: (A) cell viability assessed by ATP content; (B) histology of H&E-stained tissue sections – representative images are shown (lower panels show magnification of boxed areas in upper panels); (C) lipid droplet content, assessed by Oil Red O staining; (D) intracellular TG content; (E) ketone bodies secreted in the cell supernatants. (F) RT-QPCR analyses of genes involved in fatty acid β-oxidation (CPT1A, PGC1A, ACOX1). (G,H) RT-QPCR analyses of genes (G) and western blotting of proteins (H) involved in autophagy induction (LC3, SIRT1). (I,J) RT-QPCR analyses of pro-inflammatory interleukin genes (IL1B, TNF) (I) and of genes involved in ER stress (J) (CHOP, GADD34). Data are mean±s.e.m. of seven preparations, shown relative to vehicle. *P<0.05 versus vehicle (one-way ANOVA). Scale bars: 100 µm.