Fig. 3.

Maternal Nopp140 protein is reduced in early J11 Nopp140−/− larval brains. Central brain lobes and mid-gut tissues from w¹¹¹⁸ (wild-type) control, heterozygous J11^non-DsRed/TM3 and homozygous J11^non-DsRed larvae at day 1–2 ALH (central brain lobes A–F, polyploid gut tissues G–L) and day 4–5 ALH (central brain lobes M–R, polyploid gut tissues S–X) were immuno-stained with anti-Nopp140-RGG. Arrows in panels A and C indicate four NBs per wild-type brain lobe with large nucleoli labeled with anti-Nopp140-RGG. Nuclei were counter-stained with DAPI. n=19 (w¹¹¹⁸); n=13 (heterozygous J11^non-DsRed/TM3 larvae); n=23 (homozygous J11^non-DsRed); two technical replicates. Scale bars: 10 µm.