Fig. 4.

Embryonic and larval survival upon complete or partial loss of Nopp140. (A) Survival assays were performed for homozygous J11^DsRed or heterozygous J11^DsRed/TM3-GFP embryos, and for w¹¹¹⁸ control embryos. Freshly laid eggs were collected from well-yeasted juice plates (n=2; total number of embryos per replicate for w¹¹¹⁸: 200 and 299, and for J11^DsRed/TM3-GFP stock: 230 and 111). The number of hatched larvae were logged for the next 2 days, and the percent viable embryos was determined. Data shows the number of larvae hatched divided by the total number of embryos collected X 100%. (B) Plot shows three replicates (number of larvae per replicate: 70, 42, 62) of survival assay for homozygous J11^DsRed larvae. Newly hatched larvae were collected from a well-yeasted juice plates, and the number of living larvae were recorded in the following days until all larvae had perished. (C) Embryonic lethality and larval survivability upon Nopp140 depletion by RNAi expression using the worniu::GAL4 driver (specific for all embryonic and larval NBs) and UAS::TComC4.2 (Nopp140-RNAi line; Cui and DiMario, 2007). Compared to 86.7% of the w¹¹¹⁸ embryos, only 46.8% of the collected embryos with Nopp140 depletion (worniu-GAL4>C4.2) hatched and developed into third instar larvae, after which all larvae developed into adults (not shown). n=3; total number of embryos collected per replicate for each genotype: 200, 265 and 330; Student's t-test: two-tailed with unequal variance, P-value=0.0069.