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. 2020 Apr 28;9(4):bio046565. doi: 10.1242/bio.046565

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — MB lineage cells retain nucleolar fibrillarin under nucleolar stress. Larval brains from control w¹¹¹⁸ (wild-type), heterozygous J11^non-DsRed/TM3, and homozygous J11^non-DsRed larvae at day 3 ALH were immuno-stained with anti-fibrillarin (magenta). Image panels B, E and H show anti-fibrillarin stained central brain lobes along with mCD8::GFP (green) expressed under MB-lineage specific OK107::GAL4 driver in the same tissues. Four large nucleoli, presumably in the MB NBs, are apparent in the homozygous J11^non-DsRed larval brains (G). Arrowheads in G show small nucleoli that reside in putative GMCs. Arrows in H indicate redistributed nucleolar fibrillarin in NBs residing outside of mCD8::GFP expressing MB lineage cells (green). n=10 (wild-type); n=14 (heterozygous J11^non-DsRed/TM3 larvae); n=10 (homozygous J11^non-DsRed); two technical replicates. Scale bar dimensions are provided in panels C, F and I.