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. 2020 Apr 28;9(4):bio051367. doi: 10.1242/bio.051367

Fig. 4.

Fig. 4.

Ineffective restoration of hypomotility by co-injection of the c.875C>T variant. (A) Total swim speed of larvae injected with RC morpholinos, tbMO morpholinos, GABRA1 encoding mRNA (1000 pg/embryo), RC with GABRA1 mRNA (RC+), or tbMO with GABRA1 mRNA (tbMO+) was determined using Zebrabox technology at 5 DPF. Total number of embryos analyzed per group is depicted in the graph. (B) The total distance swam was assessed at 5 DPF using Zebrabox technology for each of the conditions in A. *P=0.036384 and #P=0.000161 and ##P=0.003010689. Representative images of larval swim patterns are depicted above panel A and B. (C) Total distance swam of larvae injected with RC, tbMO, GABRA1 c.875C>T (SDM) encoding mRNA, or tbMO with GABRA1 c.875C>T encoding mRNA (tbMO+SDM) was determined at 5 DPF. Total number of animals is indicated in the graph. ◊P=0.0153. All experiments were performed in biological triplicate and statistical analysis was performed using a standard two-tailed t-test. Error bars represent standard error of the mean of independent experiments.