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. 2020 Apr 28;9(4):bio051367. doi: 10.1242/bio.051367

Fig. 5.

Fig. 5.

Molecular and behavioral responses of gabra1 morphants. (A) QPCR was performed at 5 DPF to measure the expression of each gene indicated. Total RNA was isolated from RC injected embryos or tbMO morpholinos. Error bars represent standard deviation of biological triplicates. #P=0.0016, ##P=0.003681, §P=0.009. (B) At 5 DPF, non-injected (NI) larvae, larvae injected with RC, or larvae injected with tbMO were treated with 10 μM PTZ or no treatment (NT) control and total swim speed was assessed using Zebrabox technology. ◊P=6.74E-06, ◊◊P=5.34E-07, ◊◊◊P=1.019E-05. (C) Total distance travelled was assessed at 5 DPF in each of the groups described in B. *2.14E-05. **P=3.92141E-05, ***P=0.0002636. All experiments in B and C were performed in biological triplicate and statistical analysis was performed using a standard two-tailed t-test. Error bars represent standard error of the mean of independent experiments. For B and C, N=12/group/biological replicate.