Table 2.
MS2 and T1 L reduction during alkaline stabilization at 28°C
| Time (h) | Average viral concentration (log10 total PFU ± SE)* | Significant§ | |||||
|---|---|---|---|---|---|---|---|
| MS2 PBS control | T1 L PBS control | T1 L sludge control | MS2 sludge control | T1 L sludge stabilization | MS2 sludge stabilization | ||
| 0 | 6·98 ± 0·03 | 6·20 ± 0·16 | 6·31 ± 0·04 | 5·92 ± 0·04 | 6·31 ± 0·04 | 5·92 ± 0·04 | |
| 0·1 | –† | – | 6·28 ± 0·03 | 5·88 ± 0·06 | 1·70 ± 0·29 | 0 | No |
| 2 | – | – | 6·18 ± 0·09 | 5·71 ± 0·05 | 0·52 ± 0·26 | 0 | No |
| 12 | – | – | 6·12 ± 0·17 | 5·68 ± 0·06 | 0 | 0 | No |
| 24 | 6·76 ± 0·08 | 6·04 ± 0·06 | 6·01 ± 0·12 | 4·75 ± 0·62 | 0 | 0 | No |
| Reduction‡ | 0·22 ± 0·11 | 0·15 ± 0·22 | 0·30 ± 0·46 | 1·18 ± 0·83 | 6·31 ± 0·59 | 5·92 ± 0·04 | No |
*Average concentration of target virus ± SE from three trials under the following conditions: PBS control (virus spiked PBS, pH 7), sludge control (virus spiked in 4% TS sludge, pH 7), sludge stabilization (virus spiked into 4% TS sludge, treated with 8% (w/v) Ca(OH)2, pH 12 for 2 h then 11·5 an additional 22 h).
†Sample not tested (–).
‡Average total reduction of target virus, including loss because of experimental conditions, during three trials of stabilization ± SE.
§Significant difference between square root transformed viral concentrations of the test beakers at each time point determined by T‐HSD, P ≤ 0·05.