Table 3.
MS2 and T1 L reduction during alkaline stabilization at 4°C
| Time (h) | Average viral concentration (log10 total PFU ± SE)* | Significant§ | |||||
|---|---|---|---|---|---|---|---|
| MS2 PBS control | T1 L PBS control | T1 L sludge control | MS2 sludge control | T1 L sludge stabilization | MS2 sludge stabilization | ||
| 0 | 6·78 ± 0·09 | 6·0 ± 0·18 | 5·65 ± 0·15 | 5·99 ± 0·11 | 5·65 ± 0·15 | 5·99 ± 0·11 | |
| 0·1 | –† | – | 5·57 ± 0·14 | 5·79 ± 0·10 | 2·43 ± 0·62 | 1·63 ± 0·33 | No |
| 2 | – | – | 5·46 ± 0·13 | 5·64 ± 0·18 | 1·42 ± 0·12 | 0·75 ± 0·75 | No |
| 12 | – | – | 5·33 ± 0·08 | 5·11 ± 0·69 | 0 | 0·59 ± 0·59 | No |
| 24 | 6·72 ± 0·06 | 5·96 ± 0·18 | 5·29 ± 0·21 | 4·89 ± 0·57 | 0 | 0 | No |
| Reduction‡ | 0·06 ± 0·14 | 0·04 ± 0·36 | 0·37 ± 0·71 | 1·10 ± 1·65 | 5·65 ± 0·89 | 5·99 ± 1·79 | No |
*Average concentration of target virus ± SE from three trials under the following conditions: PBS control (virus spiked PBS, pH 7), sludge control (virus spiked in 4% TS sludge, pH 7), sludge stabilization (virus spiked into 4% TS sludge, treated with 8% (w/v) Ca(OH)2, pH 12 for 2 h then 11·5 an additional 22 h).
†Sample not tested (–).
‡Average total reduction of target virus, including loss because of experimental conditions, during three trials of stabilization ± SE.
§Significant difference between square root transformed viral concentrations of the test beakers at each time point determined by T‐HSD, P ≤ 0·05.