Table 1. Larvicidal activities of M. anisopliae derived secondary metabolites on larvae of Ae.aegypti, An.stephensi and Cx. quinquefasciatus mosquitoes at 24hr post treatment.
| Mosquito species (na = 450) | Concentration (μg/ml) | % Mortality (μg/ml) | LC50(LCL-UCL) (μg/ml) | χ 2 (df = 12) | F value |
|---|---|---|---|---|---|
| A. aegypti | Control | 4.00 | 59.83 (43.64–72.27) | 22.8* | 660.3 |
| 5 | 22.66 | ||||
| 10 | 50.66 | ||||
| 25 | 65.33 | ||||
| 50 | 73.33 | ||||
| 75 | 85.33 | ||||
| A n. stephensi | Control | 2.66 | 50.16 (48.163–54.97) | 32.9* | 748.7 |
| 5 | 33.33 | ||||
| 10 | 61.33 | ||||
| 25 | 77.33 | ||||
| 50 | 89.33 | ||||
| 75 | 97.33 | ||||
| Cx. quinquefasciatus | Control | 1.33 | 51.15 (43.90–60.43) | 19.2* | 466.1 |
| 5 | 21.33 | ||||
| 10 | 57.33 | ||||
| 25 | 64.00 | ||||
| 50 | 73.33 | ||||
| 75 | 89.33 |
na = total number of mosquito larvae used per each species, 25 larvae per replicate, three replicates were carried out, five concentrations were tested; LC50 = lethal concentration killing 50% of exposed organisms; LCL = 95% lower confidence limits; UCL = 95% upper confidence limits; χ2 = chi square (* s = significant (p < 0.05)); df = degrees of freedom.