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. Author manuscript; available in PMC: 2020 May 4.
Published in final edited form as: Cell Tissue Res. 2014 Jul 4;358(1):203–216. doi: 10.1007/s00441-014-1937-2

Fig. 2.

Fig. 2

a–u Representative immunocytochemical staining of SMC differentiation markers, neural stem cell markers and mesenchymal stem cell (MSC)-like markers (green). Rat aortic multipotent vascular stem cells (MVSCs) were isolated by explant and cultured in MVSC maintenance media before being analysed by immunofluorescence microscopy for SMC markers (a–c SM-MHC), neural stem cell markers (d–f Sox10, g–i Sox17, j–l S100β) and MSC (Mesenchymal) markers (m–o CD44, p–r CD29, s–u CD146). Nuclei were stained with DAPI (blue). Scale Bars are 50 μm. v Representative immunoblot analysis of SMC differentiation markers (SMA, CNN1, SM-MHC) and neural stem cell markers (Sox10, Sox17 and S100β) in MVSCs. Equal loading was confirmed by Ponceau S staining. Data are representative of two independent experiments