Fig. 3.

a–i Representative immunocytochemical staining (green) of SMC differentiation markers and neural stem cell markers during MVSC transition to vascular smooth muscle cells (vSMCs). MVSCs were cultured in maintenance media or differentiation media (DMEM supplemented with 10 % FBS) for 10 days before being examined for SMC differentiation markers (a, b SMA, d, e SM-MHC, g, h CNN1). Confocal immunofluorescence images for SM-MHC (c), SMA (f) and CNN1 (i). Scale Bars are 20 μm and 50 μm. j–l Representative flow cytometry analysis of Sox10 (j), Sox17 (k) and S100β (l) in MVSCs cultured in DMEM supplemented with 10 % FBS for 10 days with antibodies against Sox10, Sox17 and S100β (open curves negative IgG control cells, red filled curves cells stained with antibodies against Sox10, Sox17 and S100β). m–o Representative confocal immunofluorescence images of Sox10 (m), Sox17 (n) and S100β (o). Nuclei were stained with DAPI (blue). Bar 100 nm. Data are representative of three individual slides