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Osteogenic potential of SMCs in vitro. The ability of MSCs, MVSCs and rSMCs to differentiate to osteoblasts was determined following treatment with osteogenic differentiation media for 21 days. Osteoblast differentiation was determined by measuring calcium deposition with Alizarin Red (arrows cells positive for osteoblast characteristics). Scale bars are 25 μm. Data are representative of three independent wells
