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. 2020 May 4;15(5):e0232591. doi: 10.1371/journal.pone.0232591

Fig 3. Generation of FP receptor knockout mice.

Fig 3

A) Schematic representing strategy of Schier stop cassette insertion, single-guide RNA (sgRNA), WT Cas9 mRNA and a single-strand DNA (ssDNA) donor into the cytoplasm of C57BL/6NHsd zygotes. The ssDNA donors contained stop codons in all three reading frames to ensure a premature stop when inserted. B) Schematic representing Shier stop cassette. C) Genotyping data confirms insertion of the Schier stop cassette. D) ELISA in uterine tissue revealed diminished FP receptor expression at the protein level in mice heterozygous for the FP receptor compared to wild-type and non-detectible FP receptor expression in FP receptor knockout mice. ND = not detected. Error bars represent average ± standard deviation.