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. 2020 May 4;15(5):e0223344. doi: 10.1371/journal.pone.0223344

Fig 2. Effect of BK3C231 and 4NQO on the viability of CCD-18Co cells as assessed by MTT assay.

Fig 2

(A) Cells were treated with BK3C231 from 6.25 μM till 100 μM for 24h. An IC50 value of 99 μM was observed. (B) Cells were treated with 4NQO from 3.125 μM till 50 μM for 1h (no IC50 value), 2h (IC50 value was 41 μM), 4h (IC50 value was 24 μM), 12h (IC50 value was 34 μM) and 24h (IC50 value was 13 μM). (C) Cells were pretreated with BK3C231 at 50 μM for 2h prior to 4NQO treatment from 3.125 μM till 50 μM for subsequent 22h (no IC50 value) in comparison to BK3C231-treated (no IC50 value) and 4NQO-treated cells (IC50 value was 13 μM). Each data point was obtained from three independent experimental replicates and expressed as mean ± SEM of percentage of cell viability. * p<0.05 against negative control.