Fig 4. DNA macrolesion assessment in CCD-18Co cells using CBMN assay.

(A) Fluorescence microscopic images with acridine orange staining of mononucleated cell (i), binucleated cell (ii) and binucleated cell with micronucleus (iii). Cellular nucleus was stained green while cytoplasm was stained orange in this assay. (B) Cells were pretreated with BK3C231 from 6.25 μM till 50 μM for 2h prior to 4NQO induction at 1 μM for 2h. Each data point was obtained from three independent experimental replicates and expressed as mean ± SEM of frequency of micronucleus in binucleated cells. * p<0.05 against positive control, 4NQO only.