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. 2020 May 4;15(5):e0223344. doi: 10.1371/journal.pone.0223344

Fig 7. Nitrosative stress assessment in CCD-18Co cells.

Fig 7

(A) Determination of intracellular NO level using Orange NO probe staining. (B) Determination of extracellular NO level using Griess assay. (C) Measurement of intracellular GSH level using Ellman’s reagent. Cells were pretreated with BK3C231 at 50 μM and resveratrol (res) at 25 μM for 2h, 4h, 6h, 12h and 24h prior to 4NQO induction at 1 μM for 1h. Each data point was obtained from three independent experimental replicates and expressed as mean ± SEM of orange NO probe-stained cells (%), NO level and concentration of free thiol. * p<0.05 against positive control, 4NQO only.