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. 2020 May 4;15(5):e0231944. doi: 10.1371/journal.pone.0231944

Fig 4. Thrombin activates myosin by MLCK and ROCK.

Fig 4

(A, B) Collagen lattice assay with MLCK and ROCK inhibitors. Myometrial cells were pretreated with (A) 10 μM MLCK inhibitor ML7, or (B) 1 μM of Rho-kinase inhibitor Y-27632 for 1 h, and treated with 2 U/mL of thrombin for 30 min. Representative image (left panels) and quantification of gel areas (right graphs). n = 3 in each group. (C) Immunoblots of phosphorylated MLC2 (p-MLC2), MLC2, and β-actin of myometrial cells. (D) Immunoblots of phosphorylated MYPT1 (Thr696 and Thr853), MYPT1, and β-actin of myometrial cells. Myometrial cells were treated with 2 U/mL of thrombin as indicated time. **, p < 0.01. The experiments were repeated three times.