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. 2020 May 4;15(5):e0231944. doi: 10.1371/journal.pone.0231944

Fig 5. Effect of thrombin on PTGS2 mRNA expression and prostaglandin synthesis in myometrial cells.

Fig 5

(A, B) Thrombin-induced increases of PTGS2 mRNA at 24 h with different doses of thrombin (A), or 2 U/mL of thrombin as a function of time (B). (C-F) Thrombin-induced increases of PGF2α (C, D) and PGE2 (E, F) in the media of thrombin-treated cells. Dose-dependent changes at 24 h (C and E) and time course with 2 U/mL of thrombin (D, F). (G and H) Collagen lattice assay of myometrial cells at 30 min with 2 U/mL of thrombin, pretreated with 10 μM indomethacin for 4 h. (G) Representative image and (H) quantification of gel areas. n = 3 in each group. *, p < 0.05, and **, p < 0.01. The experiments were repeated three times.