Fig. 5.

Proteins involved in mechanotransduction are present at bleb necks. (A,B) Representative images of a 1 mg/ml collagen I-embedded MDA-MB-231 cell at t=6 h co-stained for talin and β1 integrin (talin channel shown) (A) or vinculin and β1 integrin (vinculin channel shown) (B). Scale bars: 10 µm. (C) Analysis of fluorescence intensity distribution of talin and vinculin at bleb necks as well as their co-clustering with β1 integrin at these sites. n=113 blebs for talin/β1, n=101 for vinculin/β1. Data were collected and pooled from two independent experiments. Dashed line indicates the expected incidence of blebs with co-clusters. Differences between measured and expected occurrence of co-clusters were analyzed by a t-test between percentages; **P<0.01, ***P<0.001.