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. 2020 Apr 30;133(8):jcs241976. doi: 10.1242/jcs.241976

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Thioredoxin-dependent reduction of ER-localised disulfides. (A) The redox status of Trx1 (trx) was determined by AMS differential alkylation either before (lane 1) or after (lane 2) incubation with TrxR1 and a NADPH recycling system (C). Samples not treated with AMS are shown in lanes 3 and 4. The experiment was carried out once. (B) The normalised fluorescence ratio of ER-SFGFP-iE was measured following incubation with the reduction system without Trx1 (C-Trx) or with the TrxR1 system containing wild-type Trx1 (CXXC) or active site mutants CXXS or SXXS. The experiment was repeated twice (n=3) with the average (mean±s.d.) presented.