Fig. 5.

Evaluation of the changes in the redox status of microsomal proteins following incubation with the TrxR1 system. (A) Volcano plot depicting proteins with significant changes to their redox status where the modified cysteine is localised to the ER (blue) or the cytosol (orange). Proteins identified by mass spectrometry whose redox status does not change significantly are also indicated (grey). (B) Cartoon illustrating the final cellular location of proteins whose redox status changes significantly upon incubation of microsomes with the TrxR1 system. Proteins that enter the secretory pathway are in blue whereas those that do not enter the ER are in yellow. Proteins that become more reduced are in normal text and those that become more oxidised are in italics. Note that PBXIP1 associates with the cytoskeleton and is mainly in the cytosol but it does shuttle to the nucleus to act in addition to its role as a soluble transcription factor (Abramovich et al., 2002).