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. Author manuscript; available in PMC: 2020 Sep 17.
Published in final edited form as: Anal Chem. 2019 Aug 28;91(18):11606–11613. doi: 10.1021/acs.analchem.9b01814

Table 1.

Summary of Identified QPSSSR Peptide Library after TMT0 Labeling by Maxquanta

m/z z Modified sequence Site probability Score RT (min)
448.25 2 QPSSR 52 25.95
488.23 2 QPpSSSR QPS(0.764)S(0.118)S(0.118)R 46 38.36
488.23 2 QPSpSSR QPS(0.049)S(0.759)S(0.192)R 70 26.77
488.23 2 QPSSpSR QPS(0.001)S(0.28)S(0.719)R 64 29.37
528.21 2 QPpSSpSR QPS(0.84)S(0.302)S(0.857)R 52 29.27
528.21 2 QPpSpSSR QPS(0.741)S(0.959)S(0.3)R 42 31.09
528.21 2 QPSpSpSR QPS(0.183)S(0.913)S(0.904)R 97 31.6
a

The number included in parentheses indicates the probability of phosphorylation at the indicated serine residue. The score is the Andromeda score for the best associated MS/MS spectrum. No triply phosphorylated peptides were identified.