Figure 2. a–c.

Effects of different treatments on SMMC-7721 cells apoptosis detected by flow cytometry. (a) Cells were treated with drugfree medium for 48 h and then evaluated using an Annexin V-FITC Apoptosis Detection Kit. (b) Cells were treated with 0.1mM lanthanum citrate for 48 h and then evaluated using an Annexin V-FITC Apoptosis Detection Kit. (c) Cells were treated with 15 μM KAAD-cyclopamine for 48 h and then evaluated using an Annexin V-FITC Apoptosis Detection Kit. PI, propidium iodide.