Skip to main content
. Author manuscript; available in PMC: 2020 May 4.
Published in final edited form as: Nanomedicine. 2019 Jul 23;21:102067. doi: 10.1016/j.nano.2019.102067

Figure 3.

Figure 3.

Nanoparticle in vitro characterization and MAR peptide conjugation. (A) No significant nanoparticle aggregation was observed in various media (DI-H2O, PBS, or 10% FBS in PBS) after 24 h incubation (measured using DLS). (B) PEG (10 kDa/ 20 kDa)-conjugated DIO-CA NPs had the highest repulsion ability, preventing adsorption of non-specific proteins (Bovine Serum Albumin [BSA]). BSA adsorption on DIONP formulations normalized to the number of DIONPs per batch. (C) Conjugation of FITC-LDH B peptide onto DIO-CA-PEG (10 kDa) NPs resulted in a final surface density of 33.8 μg peptide/cm2. (D) In vitro, the LC25 for RAW 264.7 macrophage cells incubated with DIONPs and SNAREs was determined to be 500 μg/ml. Heat-killed and untreated cells were used as control and treatments normalized to untreated cells.64