Figure 3. Effects of PACT/PRKRA and/or p53 on transcriptional activity by DMRT1 using luciferase reporter assay. 150 ng of DMRT1-driven firefly luciferase reporter plasmid (p4xDMRT1-luc), 3.3 ng of DMRT1 expression plasmid (pcDNA3-FLAG-DMRT1), and 10 ng Renilla luciferase vector (pRL-TK-luc) as transfection internal control in the presence or absence of PACT/PRKRA and/or p53 expression plasmids (pcDNA3-FLAG-PACT/PRKRA and/or -p53) were transiently co-transfected into 293T cells, using 1.2 μg PEI MAX. Total amount of DNA was kept at 250 ng per each transfection with pcDNA3-FLAG empty vector. 24 hours after transfection, cell lysates were used to measure luciferase activity. Relative activity is shown as the fold increase compared with the value obtained with 250 ng of pcDNA3-FLAG empty vector. The symbols -, +, and ++ indicate 0, 3.3, and 20 ng, respectively. Values are expressed as mean ± SE, n = 3. The letters above the bars indicate the results of Tukey HSD test following one-way ANOVA (p < 0.05).