Table 3. Performance of the immunohistochemical staining for ACSL3 and ACSL4 expression in liver cancer tissue microarrays for the diagnosis of HCC.

ROC curve comparisons	AUC (95% CI)	Optimal threshold	Sensitivity (%)	Specificity (%)
ACSL3, HCC vs. normal liver	0.796 (0.669–0.923)	1.29	85.8	75.0
ACSL3, HCC vs. cholangiocarcimoma	0.803 (0.624–0.963)	1.18	87.2	75.0
ACSL3, HCC vs. hepatic metastases	0.552 (0.439–0.665)	1.18	87.2	28.6
ACSL4, HCC vs. normal liver	0.967 (0.939–0.995)	0.12	93.8	93.6
ACSL4, HCC vs. CCA	0.796 (0.672–0.923)	2.77	80.1	75.0
ACSL4, HCC vs. hepatic metastases	0.801 (0.736–0.867)	13.00	62.4	94.3
Combined ACSL3 & ACSL4, HCC vs. normal liver	0.972 (0.945–0.998)	n/a	84.4	100.0
Combined ACSL3 & ACSL4, HCC vs. CCA	0.849 (0.735–0.964)	n/a	61.7	100.0
Combined ACSL3 & ACSL4, HCC vs. hepatic metastases	0.801 (0.762–0.890)	n/a	80.1	77.1

Abbreviation: n/a, not applicable.