Figure 2.

Mapping the region of BEAF that interacts with Sry-δ. (A) Schematic of the parts of BEAF that were fused to the GAL4 BD for Y2H assays. BED ZnF: 32B unique sequences, encompassing the DNA-binding BED finger (blue rectangle). LZ, purple rectangle; BESS domain, green rectangle. Numbers indicate the first and last amino acids present in the truncated proteins. (B) Results of Y2H assays, as in Figure 1A. BESS–BESS and Sry-δ-32B interactions were included as positive controls. Sry-δ interacts with M1-60. AD, activation domain; BD, binding domain; BEAF, Boundary Element-Associated Factor; LZ, putative leucine zipper; LZB, leucine zipper plus BESS; M/MID: middle region; MLZ, MID region with a putative leucine zipper; Sry-δ, Serendipity δ; Y2H, yeast two-hybrid; ZnF, zinc finger.