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. 2020 Mar 9;215(1):59–73. doi: 10.1534/genetics.120.303060

Figure 6.

Figure 6

Top2-1 binding is retained at sites of Red1 enrichment. (A) Immunofluorescence staining for Top2, Zip1, and DAPI on chromosome spreads of wild type and the top2-1 mutant at the restrictive temperature (34°) 4–5 hr after meiotic induction. Bar, 5 μm. (B) ChIP-seq analysis of Top2 and the axis protein Red1 at 34° in wild type and top2-1 mutants shown for a representative region on chromosome XII and compared to Spo11 cleavage patterns at 30° (Thacker et al. 2014) and Top2 binding at 30°. Arrows mark Top2 peaks that remain in the top2-1 mutant. These peaks show overlap with strong Red1 peaks. (C) Average Top2 binding in wild type and the top2-1 mutant at the restrictive temperature at IGRs based on orientation of genes. The 95% confidence interval for the average lines is shown.