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Leucine deprivation upregulates Redd1 and Sesn2 mRNA expression in an ATF4-dependent manner. RNA was isolated and subjected to qRT-PCR analysis for (A) Atf4, (B) Redd1, and (C) Sesn2 mRNA expression. Values are means ± SEM, n = 6. Panel A: 1-way ANOVA. Panels B and C: 2-way ANOVA. Labeled means without a common letter differ, P < 0.001. ATF4, activating transcription factor 4; REDD1, regulated in development and DNA damage responses 1.
