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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Acta Biomater. 2020 Mar 7;108:111–127. doi: 10.1016/j.actbio.2020.03.007

Figure 3.

Figure 3.

Generation of hESC-derived ONP spheroids. (A): A schematic diagram of forming hESC-derived late-stage ONP spheroids using an EZSHPERE® plate (upper row) and a phase-contrast photomicrograph of individual spheroids (see white arrow) within the plate (bottom row). (B): A schematic diagram of forming hESC-derived ONP spheroids using a 96-well Clear Round Bottom Ultra-Low Attachment Microplate® (upper row) and a low-power (left bottom row) and high-power (right bottom row) phase-contrast photomicrograph of a spheroid within the plate. (C): A phase-contrast (upper row) and an epifluorescence (bottom row) photomicrographic image of a hESC-derived ONP spheroid stained with NeuroFluor™ NeuO. (D): Immunocytochemistry on a hESC-derived ONP spheroid that was cultured for seven days with 800,000 of PODS®-hBDNF. The spheroid was stained for β-III tubulin, nestin (upper row), and PAX8 (lower row).