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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Acta Biomater. 2020 Mar 7;108:111–127. doi: 10.1016/j.actbio.2020.03.007

Figure 6.

Figure 6.

Immunocytochemistry of hESC-derived ONP spheroids that were cultured with 0.5% GrowDex®-T in vitro. (A): Schematic diagram of PODS®-hBDNF and GrowDex®-T mixture with hESC-derived ONPs spheroids in vitro. (B): A phase-contrast photomicrograph of hESC-derived ONP spheroids cultured with GrowDex®-T (gray background) and/or PODS®-hBDNF (shown in black). Human ESC-derived ONP spheroids that were cultured with 1% GrowDex®-T for 24 hours (a), 48 hours (b), and 72 hours (c), respectively. Human ESC-derived ONP spheroids that were cultured with 1% GrowDex®-T and 800,000/μL of PODS®-hBDNF for 24 hours (d), 48 hours (e), and 72 hours (f), respectively. (C): Immunocytochemistry of hESC-derived ONP spheroids cultured in NIM for 7 days. (D): Immunocytochemistry of hESC-derived ONP spheroids cultured in 0.5% GrowDex®-T for seven days. (E): Immunocytochemistry of hESC-derived ONP spheroids cultured in 0.5% GrowDex®-T with 20 ng/mL of RhBDNF for seven days. (F): Immunocytochemistry of hESC-derived ONP spheroids cultured in 0.5% GrowDex®-T with 800,000 of PODS®-hBDNF for 7 days.