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. 2020 May;61(5):735–742. doi: 10.2967/jnumed.119.234922

FIGURE 3.

FIGURE 3.

(A) Binding affinities (IC50 in nM, 1 h, 4°C; n = 3) of natGa-19F-rhPSMA-5–10 (white bars), 19F-rhPSMA-5–10 with free chelator (gray bars), and 19F-DCFPyL and 19F-PSMA-1007 (references). (B) Internalized activity of 18F-DCFPyL, 18F-PSMA-1007, and 68Ga-19F-rhPSMA-5–10 (white bars) and 18F-rhPSMA-5–10 with free chelator (gray bars), in LNCaP cells (1 h, 37°C) as percentage of the reference ligand (125I-I-BA)KuE (n = 3). (C) Lipophilicity of 18F-DCFPyL, 18F-PSMA-1007, and 68Ga-19F-rhPSMA-5–10 (white bars) and 18F-rhPSMA-5–10 with free chelator (gray bars), expressed as n-octanol/PBS (pH 7.4) partition-coefficient (log Poct/PBS; n = 6). (D) HSA binding of 19F-DCFPyL, 19F-PSMA-1007, and natGa-19F-rhPSMA-5–10 (white bars), determined on a Chiralpak HSA column. Data of reference ligands 18/19F-DCFPyL and 18/19F-PSMA-1007 were taken from a previously published study (30). Values are expressed as mean ± SD.