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. 2020 Apr 30;13:3641–3652. doi: 10.2147/OTT.S237576

Figure 3.

Figure 3

NNT-AS1 was a sponge of miR-1236-3p. (A) Predictive binding sites between NNT-AS1 and miR-1236-3p. (B and C) Dual-luciferase reporter analysis for the luciferase activities of WT-NNT-AS1 and MUT-NNT-AS1 in DDP-resistant cells was detected. (D) Relative level of miR-1236-3p in lung cancer tissues compared with adjacent normal tissues. (E) The correlation between miR-1236-3p level and NNT-AS1 level. (F and G) QRT-PCR analysis for the level of miR-1236-3p in (F) lung cancer cells and (G) DDP-resistant cells was detected. (H) The level of miR-1236-3p after transfection with NNT-AS1 in vitro. (I and J) QRT-PCR analysis for the impact of NNT-AS1 overexpression or detection on the level of miR-1236-3p in A549/DDP and H522/DDP cells were measured. *P<0.05.