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. 2020 May 4;11:2198. doi: 10.1038/s41467-020-16041-x

Fig. 5. IL-25 production from thymic tuft cells controls intrathymic iNKT2 cells.

Fig. 5

Flow cytometric detection (a) and quantitation (b) of TCRβ+PBS57:CD1d tetramer+ iNKT cells and NKT1, 2 and 17 subsets in WT (n = 8 biologically independent samples, closed symbols) and tuft cell-deficient Pou2f3−/− (n = 7 biologically independent samples, open symbols), over three indepdendent experiments. Significant P values using two-tailed unpaired t test as follows: no. of NKT2 P = 0.0033 and % NKT2 P = 0.0253. Flow cytometric detection and quantitation (c) of total iNKT and iNKT subsets in BALB/c WT (n = 9 biologically independent samples, closed symbols) and Il25−/− (n = 7 biologically independent samples, open symbols) mice, over four independent experiments. Significant P values using two-tailed unpaired t test as follows: no. of total iNKT P = 0.0013, no. of NKT2 P = 0011 and % NKT2 P = 0.0011. Flow cytometric detection (d) and quantitation (e) of total iNKT and iNKT subsets in LTβRTEC mice, 4 days after injection with either PBS (n = 8 biologically independent samples, closed symbols) or recombinant IL-25 (n = 7 biologically independent samples, open symbols), over three independent experiments. Significant P values using two-tailed unpaired t test as follows: no. of NKT2 P = 0.0482. f WT thymocyte suspensions were cultured in the presence (red line) or absence (black line) of recombinant IL-25 for the indicated period, and iNKT subsets were quantitated by flow cytometry. Data are shown as mean percentage of input (i.e. cells at d0), and error bars indicate SEM of triplicate wells from one experiment, representative of three separate experiments. All data are represented as mean ± SEM. *P < 0.05 and **P < 0.01. Source data are provided as a Source Data file.