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. 2020 May 4;11:2198. doi: 10.1038/s41467-020-16041-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a indicated that tissues from control Foxn1^Cre and LTβR^TEC mice were analysed for iNKT cell frequencies by flow cytometry. Graphs in b show cell percentages and number of iNKT cells per gram or ml of tissue, as indicated, in Foxn1^Cre (n = 7 biologically independent samples, closed symbols) and LTβR^TEC (n = 7 biologically independent samples, open symbols), over three independent experiments. Significant P values using two-tailed unpaired t test are as follows: % spleen P ≤ 0.0001, % lung P = 0.0012, % liver P = 0.0494, % blood P = 0.0383, no. of cells in the spleen P ≤ 0.0001, no. of cells in the lung P = 0.0255, no. cells in the liver P = 0.0459, no. of cells in blood P = 0.0371. c Flow cytometric analysis of intracellular IL-4 or IFNγ expression in splenic iNKT cells in Foxn1^Cre (black lines) and LTβR^TEC (red lines) mice, 2 h after i.v. administration of αGal-Cer. Grey histograms show levels of staining in non-injected mice. Percentages (d) and numbers (e) of IL-4⁺ and IFNγ⁺ splenic iNKT after αGal-Cer stimulation of Foxn1^Cre (n = 7 biologically independent samples, closed symbols) and LTβR^TEC mice (n = 8 biologically independent samples, open symbols), over three independent experiments. Significant P values using two-tailed unpaired t test as follows: no. of IL-4⁺ cells P = 0.0011, no. of IFNγ⁺ cells P = 0.0014. f shows ELISA quantitation of IL-4 (2 h post Gal-Cer injection) Foxn1^Cre (n = 7 biologically independent samples, closed symbols) and LTβR^TEC mice (n = 7 biologically independent samples, open symbols), and IFNγ (16 h post Gal-Cer injection) in serum samples of Foxn1^Cre (n = 7 biologically independent samples, closed symbols) and LTβR^TEC mice (n = 6 biologically independent samples, open symbols), over three independent experiments. Significant P values using two-tailed unpaired t test are as follows: IL-4 P = 0.0117, IFNγ P ≤ 0.0001. All data are represented as mean ± SEM. *P < 0.05, **P < 0.01, and ****P < 0.0001. Source data are provided as a Source Data file.